英语翻译nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen).RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems).nRT-qPCRs were performed on anMx3005p Real Time PCR Sy

英语翻译nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen).RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems).nRT-qPCRs were performed on anMx3005p Real Time PCR Sy
英语翻译
nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen).RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems).nRT-qPCRs were performed on anMx3005p Real Time PCR System n(Stratagene).All PCR products were verified on agarose gels nstained with ethidium bromide.We designed and synthesized nthe following primers:ACE1-sense,5′-caacatcaccacagagaccagcaa- n3′; ACE1-antisense,5′-ggtgtctcgtacatagacctccac-3′; reninsense,n5′-caatccgagaaagcctgaag-3′; renin-antisense,5′-ctgtgtcaccgtgattccac- n3′; GAPDH-sense,5′-cgagatccctccaaaatcaa-3′; and nGAPDH-antisense,5′-gtcttctgggtggcagtgat-3′

英语翻译nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen).RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems).nRT-qPCRs were performed on anMx3005p Real Time PCR Sy
RNA的提取是用Qiagen公司生产的RNeasy 迷你试剂盒.

nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe以下底漆:5′,-caacatcac...

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nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe以下底漆:5′,-caacatcaccacagagaccagcaa傻乎乎意义——n3′;5′傻乎乎反义,-ggtgtctcgtacatagacctccac-3;reninsense′,它们-caatccgagaaagcctgaag-3′′;肾素反义,5′- GAPDH-sense′-ctgtgtcaccgtgattccac n3;5′,-cgagatccctccaaaatcaa-3;nGAPDH-antisense′,5′-gtcttctgggtggcagtgat-3′

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是这样的吧？
nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe...

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是这样的吧？
nThe萃取的RNA都使用了RNeasy迷你工具包n(试剂盒)。为进行RT-qPCRs RNA表达通过使用nthe KAPA SYBR快一步法rt定量酵素聚合反应技术装备(KAPA生物系统公司)。nRT-qPCRs anMx3005p进行实时PCR系统n(Stratagene)。所有产品都是在琼脂糖PCR验证凝胶nstained与溴化乙锭。我们设计并合成nthe以下底漆:5′,-caacatcaccacagagaccagcaa傻乎乎意义——n3′;5′傻乎乎反义,-ggtgtctcgtacatagacctccac-3;reninsense′,它们-caatccgag′

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